Figure 1 | Scientific Reports

Figure 1

From: Stress and behavioral correlates in the head-fixed method: stress measurements, habituation dynamics, locomotion, and motor-skill learning in mice

Figure 1

Overview of the methods. (a) Head-fixed apparatus: the air-lifted carbon fiber container (in black) placed under the head-fixation frame on the air-dispensing base. (b) Mouse with surgically attached head-plate fixed to the head-plate holder in the head-fixation frame. (c) Mouse wrapped in flannel for transfer between the home cage and the head-fixed apparatus. (d) Mouse partially restrained with a paper cup covering its body with the tail kept outside of the cup ready for blood sampling collection with a glass capillary after a tail-snip with a razor blade. (e) Video frame from the camera mounted on the top of the head-fixed apparatus to collect information for the locomotion analysis. Red arrow points at the yellow sticker attached to the floating container that was tracked with the EthoVision software for the movement analysis. (f) Example of heatmaps (top panel) and track maps (bottom panel) extracted with the software for locomotion analysis from the recorded videos at day 1, day 15 and day 25.

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