Figure 5

Locomotion dynamics during the 120-min head-fixed session. (a–g) Movement and distance analysis, averages from all the 25 head-fixed sessions. (a, b) Individual differences between the head-fixed animals in the absolute movement time (proportion of frames with movement recorded at 30 Hz resolution) and in the minutes with movement per head-fixed session (when counting every minute with any movement), respectively; dashed line represents the grand average from all the sessions and all the animals. (c) Average distance traveled during every minute of the 120-min head-fixed session, all days combined; dashed line corresponds to the grand average from all the sessions and all the animals combined. (d–g) Total distance traveled and the movement time during the first and second hours of the head-fixed session calculated with a resolution of seconds. (d, e) Individual animal data for the total distance traveled and for the movement time. (f, g) No statistically significant change in the total distance traveled and in the average movement time between the first and the second hour of the head-fixed session (paired t-test, p = 0.0882 and p = 0.0514, respectively). Outliers identified at 2nd hour (black filled squares at 141.643 and 177.890 m) but included in data analysis (removal would change the statistical outcome to significant drop in distance traveled during the 2nd hour of the session with p = 0.0222); n = 8.