Figure 7

Spinning behavior analysis in the 5-day head-fixed protocol. (a) Example of locomotor activity measured with the MHC Tracking System (top panels) and with the EthoVision software (bottom panels). In the MHC Tracking System, the additional option of colorometric presentation was available (from blue colors to red colors reflecting the passing time of the session). Note the visible difference between the two methods. In the MHC Tracking System analysis, the lines represent movement of the container relative to the mouse. In the EthoVision software analysis – movement of the yellow sticker attached to the container. Analysis of spinning behavior in the context of a preferred placement of the container was possible only with the MHC Tracking System. Mice shifted the container from the middle to the side throughout the session and ran in proximity to the walls, once their control over the container was refined. (b) Zone preference data from all training days (D1–5) recorded from 4 animals presented as the average percentage of the time spent in the zone during the 60-min head-fixed session. Schema of the zone division of the floating container (red corresponds to the walls and grey to the middle part of the container).