Figure 3 | Scientific Reports

Figure 3

From: Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment

Figure 3

Phenotypic changes in planktonic BCG cells derived from increased expression of dosR, BCG0114, BCG0642c, ethR, and BCG3766c. Ziehl–Neelsen staining of the different BCG strains sampled from mid-log phase planktonic cultures in 7H9 OADC 0.05% Tween 80 (a). Growth (as OD600nm readings) of each recombinant BCG was compared with that of parental BCG harboring the empty vector pMV361 (BCG WT::pMV361) (b). Two-Way ANOVA followed by Dunnett’s multiple comparison test was used to compare apparent growth, using four biological duplicates; p values for the recombinant strains compared with BCG::pMV361 being described in results. Bacterial replication (CFU/mL) (c) was compared by 2-Way ANOVA followed by Tukey’s multiple comparison test, using four biological duplicates. Each experiment was repeated independently two times, and one representative result is shown. Brackets encompass the comparisons for which statistically significant p values are shown on top of the bars depicting the means.

Back to article page