Figure 2
From: Der p 1-based immunotoxin as potential tool for the treatment of dust mite respiratory allergy
In vitro functional characterization of the allergen and toxin domains. (A) Comparison between nDer p 1 and proDerp1αS cysteine protease activities by mean of Boc-QAR-AMC fluorogenic peptide assay, in the absence or presence of E64 cysteine protease inhibitor. (B) Rabbit reticulocytes assays was used for testing the ribonucleolytic activity of α-sarcin within proDerp1αS. α-Fragment release, highlighted by a white arrow, indicates the specific SRL cleavage. Equimolar amounts (3, 6, 12 pmol) of α-sarcin and immunotoxin were tested and compared to ribotoxin-free control, C.
