Figure 5
Effect of I. obliquus and G. jasminoides extracts on Nile red fluorescence (A, C) and survival (B, D) of C. elegans. Nile red assay (A, C): Vital Nile red fluorescence of C. elegans treated with control, AICAR (100 μM) and extracts of I. obliquus (A) and G. jasminoides (C). Bars represent the mean fluorescence intensities of at least three independent experiments expressed as % of control worms ± SD. Significance was assessed by One-Way ANOVA and Bonferroni post-test (***p < 0.001; **p < 0.01; *p < 0.05). Survival assay (B, D): Worms were treated with control, reserpine (30 µM) and extracts of I. obliquus (B) and G. jasminoides (D). Bars represent the mean DT50 ± SD of three parallel experiments. Significance was assessed by One-Way ANOVA and Dunnett’s post-test (*p < 0.05; **p < 0.01).
