Figure 5

Cysteine replacements with alanines in D2L5 extracellular loops of LCa_v3 channels alter the relative passage of differing divalent cations, Ba²⁺ or Sr²⁺ relative to Ca²⁺. (A) Representative current traces of peak barium (Ba²⁺) and calcium (Ca²⁺) currents normalized to the size of peak Ca²⁺ currents. Current–voltage relationships of the fold change in peak (B) Ba²⁺ and (C) Sr²⁺ current size normalized to peak Ca²⁺ current levels. Graphs of the fold change in peak current sizes for (B) Ba²⁺ and (C) Sr²⁺ compared to Ca²⁺ currents. Graphs in (B,C) are illustrated with mean ± SEM with replicates (n) indicated by grey diamonds. Fold change in LCa_V3–12a and LCa_V3–12b T-type channel currents are inverted when cysteines replaces alanines in D2L5 extracellular loops (LCa_v3 Δcys), where Ca²⁺ currents are larger instead of smaller than Ba²⁺ or Sr²⁺ currents. Data contained in this figure were analyzed and illustrated using OriginPro 2018 (64-bit) SR1 b9.5.1.195.