Figure 8
Swapping of snail and human D2L5 and D4L6 extracellular loops can generate high Na+ current passing human Cav3.2 channels and high Ca2+ current passing snail LCav3 channels. Representative traces (top panels) and graphs (bottom panels) illustrating the fold increases in peak currents when equimolar 135 mM [Na]ex replaces 135 mM [NMDG+]ex in presence of 2 mM [Ca2+] for D2L5 and D4L6 extracellular loop mutants in (A) snail LCav3 (12a/12b) background, and (B) human Cav3.2 background. Graphs illustrates mean ± SEM with replicates (n) illustrated by grey diamonds. Data to generate graphs were compared in a parametric one-way ANOVA with statistical significance evaluated in Tukey post hoc analyses. In graphs, data are not significant, unless stated, where **p < 0.01. Statistical significances for (A,B) are illustrated in Supplementary Tables S4 and S5. Color coding of differing Cav3 channels: Cav3.1 (light blue), Cav3.2 (dark blue), Cav3.3 (green), LCaV3–12b (orange), LCaV3–12a (red), LCav3 Δcys mutants (striped orange or red bars), snail LCav3 or human Cav3.2 channels with chimeric extracellular loops (light purple). Data contained in this figure were analyzed and illustrated using OriginPro 2018 (64-bit) SR1 b9.5.1.195.
