Figure 9
Normalized peak currents in response to voltage steps generated in conditions of extracellular 4 mM [Ca2+] and 100 mM intracellular Li+ (A), or 100 mM intracellular Na+ (B), with reverse potentials and calculated relative permeabilities shown graphically. Note the differing reversal potentials and slope conductances of outward currents in the current–voltage relationships for (A, Li+) and (B, Na+), and a characteristic uniformity of voltage-dependent inward Ca2+ currents peaking at more hyperpolarized voltages for wild type and extracellular loop chimeras of snail LCav3 T-type channels, compared to human Cav3.1, Cav3.2 and Cav3.3 channels (see Fig. 1 for biophysical description of wild type snail LCav3 and human Cav3.x channels). Graphs illustrate mean ± SEM with replicates (n) illustrated by grey diamonds. Data to generate graphs were compared in a parametric one-way ANOVA with statistical significance evaluated in Tukey post hoc analyses. Data displayed in graphs are not significant, unless stated, where **p < 0.01. Statistical significances for (A,B) are illustrated in Supplementary Tables S6 and S7, respectively. Color coding of differing Cav3 channels: Cav3.1 (light blue), Cav3.2 (dark blue), Cav3.3 (green), LCaV3–12b (orange), LCaV3–12a (red), LCav3 Δcys mutants (striped orange or red bars), snail LCav3 or human Cav3.2 channels with chimeric extracellular loops (light purple). Data contained in this figure were analyzed and illustrated using OriginPro 2018 (64-bit) SR1 b9.5.1.195.
