Figure 5

MYC plays a key role in IL-21-induced proliferation in Farage cells and the primary cells. (a) An RNA-seq gene expression classifier distinguishes germinal centre B cell-like (GCB) DLBCL, unclassified (UC) DLBCL and activated B cell-like (ABC) DLBCL. The ABC- and GCB-specific scores were computed for each sample by averaging the z-scores for ABC and GCB genes, respectively. (b) Gene expression profile of DLBCL genetic drivers in EBV-positive DLBCL after IL-21 treatment. Statistical analysis was performed using FDR-based q values, and all data shown were significant with a q value of < 0.01. The arrow indicates a common upregulation of MYC in both groups after IL-21 treatment. (c) The MCODE plugin of Cytoscape was used to extract subnetworks in STRING-based network analysis for co-DEGs in Farage cells and primary cells. Cluster 3 was generated from the entire picture, and the core module was extracted from cluster 3 by disabling the Fluff function in MCODE. The nodes in blue indicate genes involved in cell cycle regulation (GO: 0051726, FDR < 0.0001), and the nodes in red indicate genes involved in the G1/S transition in the mitotic cell cycle (GO: 000082, FDR < 0.0001). The arrow indicates a central position of MYC in the core module. PTM, posttranlational modification; TR, translational regulation.