Figure 2 | Scientific Reports

Figure 2

From: SIRT7 deficiency suppresses inflammation, induces EndoMT, and increases vascular permeability in primary pulmonary endothelial cells

Figure 2

Effects of SIRT7 silencing on mRNA levels of inflammatory mediators in human and murine primary pulmonary endothelial cells. SIRT7, ICAM1, VCAM1, IL6, or IL8 mRNA levels were measured by RT-qPCR and normalized to GAPDH mRNA levels in HPAEC cultures (A) or to β-actin mRNA levels in endothelial cell cultures from wild type or Sirt7+/− mice (B). Data are expressed as fold changes relative to average mRNA levels in unstimulated (LPS 0) cell cultures transfected with CTRL siRNA (A) or unstimulated cell cultures from wild type mice (B). In (A), bars represent averages and brackets standard deviations of measurements for two samples per condition for CTRL-silenced (□) and SIRT7-silenced (■) cell cultures. Experiments were performed on at least three separate occasions with similar results. In (B), bars represent averages and brackets standard deviations of cell cultures from three wild type (□) and three Sirt7+/− (■) mice. Stars denote significant differences (P < 0.05) between CTRL- and SIRT7-silenced or wild type and Sirt7+/− endothelial cell cultures at each LPS concentration. P values were calculated using the Student’s t test.

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