Figure 3 | Scientific Reports

Figure 3

From: SIRT7 deficiency suppresses inflammation, induces EndoMT, and increases vascular permeability in primary pulmonary endothelial cells

Figure 3

Effects of SIRT7 silencing on protein levels of inflammatory mediators in human primary pulmonary endothelial cells. ELISA measurements of soluble ICAM1, IL6, or IL8 protein levels in media from CTRL- or SIRT7 silenced HPAEC (A) and HPMVEC (B). WB measurements of SIRT7, ICAM1, VCAM1, or total NFκB protein levels in cell lysates from CTRL- or SIRT7-silenced HPAEC (C) and HPMVEC (D). Data shown are for two replicate samples per condition for CTRL siRNA and one sample per condition for two different strands of SIRT7 siRNA. Different proteins for the same group of samples, either from the same membrane or different gel and membrane, are demarcated by white spaces. Full-length blots are presented in Supplementary Figures 1 and 2. Densitometry values for ICAM1 and VCAM1 (C) and VCAM and NFκB (D) relative to α-tubulin are shown below the respective WB. In all panels, bars represent averages and brackets standard deviations of measurements for two samples per condition for CTRL-silenced (□) and SIRT7-silenced (■) cell cultures. Stars denote significant differences (P < 0.05) between CTRL- and SIRT7-silenced cell cultures at each LPS concentration. Experiments were performed on at least three separate occasions with similar results. P values were calculated using the Student’s t test.

Back to article page