Figure 4 | Scientific Reports

Figure 4

From: SIRT7 deficiency suppresses inflammation, induces EndoMT, and increases vascular permeability in primary pulmonary endothelial cells

Figure 4

Effects of SIRT silencing on NFκB signaling. (A) Total NFκB protein in cytoplasmic (“C”) and nuclear (“N”) fractions of CTRL- or SIRT7-silenced HPAEC 6 h after LPS stimulation. α-tubulin and HDAC2 were used to control for sample loading of cytoplasmic and nuclear fractions, respectively. Ratios of nuclear to cytoplasmic NFκB for each condition are designated in the panel for NFκB. (B) Phosphorylated NFκB protein levels in CTRL- or SIRT7-silenced HPAEC 2 h after LPS stimulation. Densitometry values for phosphorylated NFκB relative to α-tubulin (RDU) are shown. Different proteins for the same group of samples are demarcated by white spaces.

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