Table 1 Primers used for quantitative RT-qPCR.

From: SIRT7 deficiency suppresses inflammation, induces EndoMT, and increases vascular permeability in primary pulmonary endothelial cells

Gene

Primer sequencesa

Human SIRT7

ACGCCAAATACTTGGTCGTCT

AGCACTAACGCTTCTCCCTTT

Human ICAM1

TTGGGCATAGAGACCCCGTT

GCACATTGCTCAGTTCATACACC

Human VCAM1

CAGTAAGGCAGGCTGTAAAAGA

TGGAGCTGGTAGACCCTCG

Human IL6

CCTGAACCTTCCAAAGATGGC

TTCACCAGGCAAGTCTCCTCA

Human IL8

TGACTTCCAAGCTGGCCGTGG

ACTGCACCTTCACACAGAGCTGC

Human GAPDH

ATGGGGAAGGTGAAGGTC

GGGGTCATTGATGGCAACAATA

Mouse Sirt7

ACCACTGCCTTACCTCACTC

CTGTGCCGCATACCCAATAC

Mouse Icam1

CTGCCTCTGAAGCTCGGATA

GTCACCTCTACCAAGGCAGT

Mouse Vcam1

ACGAGGCTGGAATTAGCAGA

TCGGGCACATTTCCACAAG

Mouse IL6

CCGGAGAGGAGACTTCACAG

TCCACGATTTCCCAGAGAAC

Mouse β-actin

GTTGGAGCAAACATCCCCCA

CGCGACCATCCTCCTCTTAG

Mouse Gapdh

AATGTGTCCGTCGTGGATCT

AGACAACCTGGTCCTCAGTG

  1. aFor each primer set, top sequence denotes forward primer and bottom sequence reverse primer
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