Figure 6 | Scientific Reports

Figure 6

From: Sodium-glucose co-transporter (SGLT) inhibitor restores lost axonal varicosities of the myenteric plexus in a mouse model of high-fat diet-induced obesity

Figure 6

Myenteric plexus of the mouse small intestine stained by the whole-mount preparation method. Double immunofluorescence staining for synaptophysin (green) and c-Kit (red) in STD-Veh (a–c), HFD-Veh (d–f), and HFD-PLZ mice (g–i). Nuclei were counterstained with DAPI (c,f,i, blue). Synaptophysin immunoreactivities in STD-Veh (a,c, white arrows) and HFD-PLZ mice (g,i, white arrows) were clearly observed as numerous small granular immunoreaction signals in the myenteric plexus. (j) In HFD-Veh mice (n = 3), the synaptophysin density index (stained area/total myenteric area) was lower than that in STD-Veh mice (n = 3), but it was significantly increased in HFD-PLZ mice (n = 3). (k) Graphical summary of the model. Results are presented as the mean ± SD. Statistical significances were calculated by ANOVA followed by Bonferroni post-hoc tests. **P < 0.01, ***P < 0.001. Bars: 10 µm.

Back to article page