Figure 5
From: β1 integrin, ILK and mTOR regulate collagen synthesis in mechanically loaded tendon cells
Role of mTOR pathway in regulation of collagen expression. (a) Immunoblot analysis of tendon-derived stromal cells after incubation with AKT inhibitor (GSK2141795) and mTOR inhibitors (INK128, PP247 and Torin) for 48 h. (b) In-cell western analysis of human tendon cells after 48 h and 72 h incubation with GSK2141795 and mTOR inhibitors. (c) Densitometry analysis of the in-cell western in b. (d–g) qRT-PCR analysis of gene expression in human tendon cells after 48 h of incubation with GSK2141795 (d), INK128 (e), Torin (f), and PP247 (g). Increasing ΔCt indicates decreasing gene expression. Two-way and one-way ANOVA followed by Bonferroni's multiple comparisons test for data of in-cell western densitometry and qPCR, respectively.; mean ± SE; ns P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001; n ≥ 3 biological replicates.
