Figure 1 | Scientific Reports

Figure 1

From: A putative UDP-glycosyltransferase from Heterorhabditis bacteriophora suppresses antimicrobial peptide gene expression and factors related to ecdysone signaling

Figure 1

Hb-ugt-1 is upregulated in response to host-associated factors, but not development-inducing symbiotic bacteria. The expression of Hb-ugt-1 in response to assorted host factors and Photorhabdus luminescens bacteria was assessed by qPCR at the indicated time points. Approximately 1,000 axenic infective juveniles (IJs) were exposed to 25% Drosophila melanogaster line Oregon-R (A) or Manduca sexta (C) hemolymph plasma while 5,000 nematodes were injected directly into the hemocoel of live Galleria mellonella larvae (B). One thousand IJs were plated on a lawn of P. luminescens as a virulence-neutral control (D). Average fold change drawn from three independent trials and calculated according to the 2−ΔΔCT method is shown, where error bars represent standard error (*p < 0.05, **p < 0.01, ***p < 0.001). Graphs were generated using GraphPad Prism version 5.03 (www.graphpad.com).

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