Figure 2

Hb-ugt-1 is a putative UDP glycosyltransferase (UGT). (A) The amino acid sequence for Hb-ugt-1 displays sequence similarity to ecdysone glycosyltransferases. A region containing the domain associated with sugar binding is shown, where color intensity represents percent identity. The full alignment was produced with sequences from three insects; Drosophila melanogaster (NP_001246082.2), Bombyx mori (NP_001243972.1), and Manduca sexta (XP_030029659.1), three nematodes; Ancylostoma ceylanicum (EYC08379.1), Brugia malayi (XP_001894161.1), and Caenorhabditis elegans (NP_500913.1), and three insect-infective viruses; Spodoptera frugiperda multiple nucleopolyhedrovirus (AAP79109.1), Agrotis ipsiolon nucleopolyhedrovirus (YP_002268062.1), and Autographa californica nucelopolyhedrovirus (NP_054044.1). (B) A maximum-likelihood phylogenetic tree was generated for the aligned sequences with MEGA-X software. The consensus tree shown was developed from 200 bootstrap replicates and numbers next to branches indicate the percentage of replicates in which the associated taxa clustered together. The alignment image was produced with Jalview version 2.11.1.0 (www.jalview.org) and the image for the phylogenetic tree was exported from MEGA-X Version 10.1.5 (megasoftware.net).