Figure 3
The effects of BDNF on B-CLL pro-survival signaling are similar to those of BAFF, APRIL, and CXCL12 combined. (a) Representative western blot showing expression of NTSR2, p-Src, Src, and Bcl-2 by B-CLL cells isolated from patients. Cells were either co-cultured for 48 h with autologous NLC or cultured alone in complete medium supplemented (as indicated) with exogenous human (h) CXCL12 (100 ng/mL), BAFF (2 ng/mL), APRIL (25 ng/mL), or BDNF (100 ng/mL). (b,c,d), full-length blots are shown in the Supplementary Fig. S7. Quantification of NTSR2 (b), p-Src (c), and Bcl-2 (d) expression in four different patient samples. Data are presented as the mean ± SEM from at least three independent experiments, (*p < 0.05, **p < 0.01). Blots are cropped for clarity; full blots are shown in the Supplementary Fig. S7.
