Figure 2 | Scientific Reports

Figure 2

From: Targeting the PLK1-FOXO1 pathway as a novel therapeutic approach for treating advanced prostate cancer

Figure 2

PLK1-mediated phosphorylation of FOXO1 leads to its nuclear exclusion in DU145 cells. (a) Representative images of the cellular localization of Flag-tagged FOXO1 WT and FOXO1 mutants (S75A and S75D) in DU145 cells immunostained for Flag (green) and Dapi (blue) (left). Quantification of a representative experiment is shown in the bar graph (right). Similar results were obtained from 3 independent experiments. Scale bar = 10 μm. (b) DU145 cells expressing either empty vector (EV), Flag-tagged FOXO1 WT, or a mutant (S75A or S75D) were also subjected to subcellular fractionation. The levels of exogenous FOXO1 in total (WCL), nuclear (N), and cytoplasmic (C) fractions were determined by Western blotting with anti-Flag antibody. Lamin A/C and α-tubulin were used as a nuclear marker and a cytoplasmic marker, respectively.

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