Figure 6
BI2536 in combination with nocodazole induces apoptosis in advanced PCa cells. (a) DU145 cells were treated with BI2536 and NoC for 72 h as indicated. Cells lysates were prepared and subjected to western blot anaysis with the indicated antibodies. GAPDH was used as an internal loading control. (b) DU145 cells were treated with BI2536 or NoC alone or in combination (combo). Cells were stained with Annexin V-FITC and PI 72 h post-treatment. Apoptotic cells were analyzed by flow cytometry. Graph represents the percentage of apoptotic cells as mean ± SD of 3 independent experiments.
