Figure 1
The introduction of DiI analogues to liposome-mediated vessel painting provides greater wavelength flexibility. (a) Representative confocal images of cerebral vasculature labelled by liposome-mediated vessel-painting. Maximum projections of optical sections from the brain surface (N = 1 for Neuro-DiO(C14) and N = 2 for other dyes; each image is a representative of more than three observations). (b) Absorption spectra of DiI and its analogues. The right panel is spectrum of the shorter wavelength region of the spectrum in the left panel showing. These measurements were performed using solutions of 2 μM (left panel) and 10 μM (right panel) in ethanol. (c) Representative two-photon microscopy of the cerebral vasculature. Maximum projections of optical sections from the brain surface (N = 1 for each dye; each image is a representative of three observations). Scale bars = 200 μm (a) and 50 μm (c).
