Figure 2 | Scientific Reports

Figure 2

From: Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures

Figure 2

Vessel painting is applicable to various organs. (a–d) Two-photon microscopy of DiR(C18)-labelled blood vessels in lung (a), liver (b), intestine (c), and kidney (d) tissues. XY maximum projections of optical sections from sample surfaces (N = 2; representative images of more than six observations). Scale bars indicate 50 µm (a–c) and 10 µm (d). (e–f) Comparison of labelling patterns among vessel painting, fluorescently-labelled tomato lectin, and anti-CD31 antibody for renal vasculature. Representative confocal images of liposome-mediated vessel painting with DiI(C12) [N = 2 for DiI(C12) and tomato lectin, N = 1 for CD31; each image is a representative of more than five observations] (e), Tomato lectin (f), and anti-CD31 antibody (g). Maximum projections of optical sections from sample surfaces. Scale bar = 100 µm (e–f).

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