Figure 5 | Scientific Reports

Figure 5

From: Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures

Figure 5

Glomeruli can be labelled with various probes after vessel painting. Confocal microscopy of quadruple-labelling of renal tissues multi-labelled with vessel painting and other probes. (a) XY maximum projection of confocal optical sections taken from the surface of a renal slice. (b) A confocal optical section of a glomerulus. F-actin (magenta), a-tubulin (yellow), endothelium (grey), and nuclei (cyan) are labelled with rhodamine-phalloidin, DM1A Alexa 488 conjugate, DiD(C18), and DAPI, respectively. (N = 2; each image is a representative of 19 observations.) Scale bars = 100 μm (a) and 10 μm (b).

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