Figure 1

RB inhibits the Tau aggregation in vitro. (A) The domain organization of Tau. Tau is a natively unfolded protein having two domains, projection domain and a microtubule-binding domain. Rose Bengal is an anionic Xanthene dye widely used in clinical diagnosis. (B) The aggregation kinetics demonstrating a reduction in ThS fluorescence in samples incubated with various concentrations of RB. 40 µM RB showed maximum inhibition of Tau aggregation. (C) The graph showing the percentage of aggregation inhibition at the end of 72 h. (D) CD spectroscopy of RB treated samples, 40 µM RB and 20 µM RB observed to induce conformational changes in Tau aggregates. (E) The electron microscopy images of the RB treated sample have small broken fragments of Tau while the untreated sample has long tangled filaments.