Figure 1
From: Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells
Total RNA sequencing of resected neonatal hearts. (A) Experimental design scheme for total RNA sequencing. Cardiac apex resection or false surgery was performed in 1-day-old and 7-day-old rats, and samples were collected one (D1) or five (D5) days after the procedure. We created this figure in the Mind the Graph platform (www.mindthegraph.com). (B) Only the apex portion (1/3 of the heart) was considered for the experiment; in the PCA diagram, samples were clustered considering three main parameters: age at time of procedure, procedure and time post-procedure. The more prevalent responses were age at time of procedure (x axis, corresponds to 42% of the variance) followed by time post-procedure (y axis, corresponds to 19% of the variance). (C) Biological processes and pathways related to the inflammatory response and cellular movement in comparison to their respective sham-operated groups at different time points (R1D1/S1D1; R1D5/S1D5; R7D1/S7D1 and R7D5/S7D5) through ingenuity enrichment analysis. Red and blue bars show activation and inactivation of pathways, respectively, showing that the overall inflammatory responses are contrasting between neonatal development times.
