Figure 2
From: Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells
Macrophage infiltration after apex resection. (A) Macrophage infiltration evaluated (mean ± SD) by CD68-positive cell staining in the apex areas of R1D1 (n = 7), R1D5 (n = 7), R7D1 (n = 7) and R7D5 (n = 6). (B) Bars show total CD68-positive cells normalized by heart tissue area, considering that non-resected rats do not display macrophage infiltration in the apex area and that CD68 infiltration increases with time. Values are relative to P1D1. ns = non-significant; *p-value ≤ 0.05 and ***p-value ≤ 0.001. (C) Adult bone marrow-derived macrophages cultivated for 7 days were stained for CD68 and then polarized towards M1 and M2 profiles. (D) The gene expression pattern of polarized macrophages was analysed by real-time PCR. M1 polarized macrophages were confirmed for IL-1b, TNF-a and NOS2 gene expression (black bars), and M2 polarized macrophages were confirmed for Arginase 1, CCL2 and TGF-b (grey bars). Scale bars = 50 µm e 100 µm.
