Table 1 List of primers used for macrophage polarization and myofibroblast differentiation assessment through RT-PCR.

From: Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells

Gene

Sense Primer (5′-3′)

Antisense Primer (5′-3′)

IL-1β

CCTGTGTGATGAAAGACGGC

TATGTCCCGACCATTGCTGT

TNF-α

ATCGGTCCCAACAAGGAGG

GATAAGGTACAGCCCCATCTGC

NOS2

CCTGTGTTCCACCAGGAGAT

CACCAAGACTGTGAACCGGA

Arg1

TCCAAGCCAAAGCCCATAGA

AGCTTTCCTTAATGCTGCGG

TGF-β

TGAGTGGCTGTCTTTTGACG

CAGGAAGGGTCGGTTCATGT

CCL2

CACAGCTGCTGCTTTCACC

GATGGGCTTCAGCACAGACT

α-SMA

TGGAAAAGATCTGGCACCAC

CTCCGTTAGCAAGGTCGGA

Col1

CATGTTCAGCTTTGTGGACCT

TCCACGTCTCACCATTAGGG

Col3

AGCTTTGTGCAATGTGGGAC

ATCACAGAGGACAGATCGGA

  1. M1 macrophages were confirmed by IL-1β, TNF-α and Nos2 expression, and M2 macrophages were assessed for Arginase 1 (Arg1), CCL2 and TGF-β expression. For fibroblast differentiation and secretion, we used α-SMA, Col1 and Col3 expression.
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