Figure 2

Histological observation of gonadal anlagen and PGCs in serial transverse sections of 3 dah piwil2 mutants induced by CRISPR/Cas9. Three different phenotypes are shown: (A,B) type A, no gonadal anlagen and PGCs; (C,D) type B, morphologically atrophic and/or locally restricted PGCs; and (E,F) type C, gonadal anlagen and PGCs similar to control. Arrowheads indicate gonadal anlagen. Scale bar = 50 µm.