Table 1 PCR conditions used in this study.

From: Evaluation of PCR conditions for characterizing bacterial communities with full-length 16S rRNA genes using a portable nanopore sequencer

PCR condition Trial 0 (T0)

98 °C (Preheating)

2 min

35 cycles

98 °C (Denaturation)

10 s

60 °C (Annealing)

15 s

68 °C (Extension)

2 min

PCR condition Trial 1 (T1)

98 °C (Preheating)

2 min

35 cycles

98 °C (Denaturation)

10 s

60 °C (Annealing)

15 s

68 °C (Extension)

3 min

PCR condition Trial 2 (T2)

98 °C (Preheating)

2 min

35 cycles

98 °C (Denaturation)

10 s

60 °C (Annealing)

1 min

68 °C (Extension)

2 min

PCR condition Trial 3 (T3)

98 °C (Preheating)

2 min

35 cycles

98 °C (Denaturation)

10 s

60 °C (Annealing)

1 min

68 °C (Extension)

3 min

PCR condition Trial 4 (T4)

98 °C (Preheating)

2 min

35 cycles

95 °C (Denaturation)

1 min

60 °C (Annealing)

1 min

68 °C (Extension)

3 min

  1. Conditions that differ from T0 are shown in bold italic style.
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