Figure 4
Fluorescence dynamics of eGFP knockdown over time. (A) Representative fluorescence images of embryonic and polyp stages are shown for each selected timepoint and condition. For clarity, embryos and polyps are outlined with dashed lines in all cases where eGFP fluorescence is not obvious. Scale bar 100 μm. (B) Fluorescence quantification over time. ~ 50% of NE control animals started appearing fluorescent by 1 dpf, and their fluorescence levels reach an upper threshold (fluorescence saturation) by 4 dpf. In contrast, the eGFP shRNA mixture-electroporated animals show very low fluorescence levels throughout embryonic development and during the first 2 days of primary polyp growth. At the 6 dpf timepoint, the fluorescence levels start to increase in ~ 50% of the polyps, and it took up to 28 dpf for the fluorescence levels to be equivalent to the NE control. Dots represent mean values and error bars show standard deviations (n = 10–16 for embryonic samples; n = 20–50 for polyp samples). A.U. arbitrary units.
