Figure 7 | Scientific Reports

Figure 7

From: α-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages

Figure 7

Cytokine IL-1β release during α-hemolysin stimulation along with mitoROS inhibition and blockage of potassium efflux. (A) ELISA was performed to assess IL-1β release in cell culture supernatants during HlyA stimulation, along with mitochondrial ROS inhibitor (MitoTEMPO 20 μM) and potassium efflux inhibition (140 mM KCl and Glibenclamide 100 μM). Data is represented as IL-1β concentration in pg/ml (Mean ± SEM). For statistical analysis, one-way ANOVA with Bonferroni’s test for multiple comparisons was used. P value is shown as ***p ≤ 0.001. (B) Immunoblot showing the cleaved form of IL-1β in acetone precipitated culture supernatants.

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