Figure 2
Age-dependent change of integrins. Immunofluorescence staining of β1 integrin (green) and α6 integrin (red) in 30s sun-protected Caucasian skin (A), 60s sun-protected Caucasian skin (B), 30s sun-exposed Caucasian skin (C) and 60s sun-exposed Caucasian skin (D) [numbers indicate the age range (decade) of the skin donors]. Immunofluorescence staining of β4 integrin (green) and α6 integrin (red) in 30s sun-protected Caucasian skin (F), 60s sun-protected Caucasian skin (G), 30s sun-exposed Caucasian skin (H) and 60s sun-exposed Caucasian skin (I). β1 integrin’s signal intensity (E) and β4 integrin’s signal intensity (J) were analyzed using WINROOF 2013 image analyzing software (Mitani, Fukui, Japan, https://www.mitani-visual.jp/products/image_analys_ismeasurement/winroof/). mRNA expression levels of ITGB1 (K) and ITGB4 (L) were analyzed by qPCR. Data are expressed as mean ± SD from each of 5 donors in I and J. *p < 0.05, **p < 0.01. Bars: 100 μm.
