Figure 3
Age-dependent change of laminins. Immunofluorescence staining of laminin-α5 in 30s sun-protected Caucasian skin (A), 60s sun-protected Caucasian skin (B), 30s sun-exposed Caucasian skin (C) and 60s sun-exposed Caucasian skin (D) [numbers indicate the age range (decade) of the skin donors].Immunofluorescence staining of laminin-α3 in 30s sun-protected Caucasian skin (F), 60s sun-protected Caucasian skin (G), 30s sun-exposed Caucasian skin (H) and 60s sun-exposed Caucasian skin (I). Laminin α5′s signal intensity (E) and laminin α3′s signal intensity (J) were analyzed using WINROOF 2013 image analyzing software (Mitani, Fukui, Japan, https://www.mitani-visual.jp/products/image_analys_ismeasurement/winroof/). mRNA expression levels of LAMA5 (K), LAMB1 (L), LAMA3 (M) and LAMB3 (N) were analyzed by qPCR. Bars: 100 μm. Data are expressed as mean ± SD of three independent experiments from each of 5 donors in I–L. *p < 0.05.
