Figure 3 | Scientific Reports

Figure 3

From: Inhibition of acetyl-CoA carboxylase by spirotetramat causes growth arrest and lipid depletion in nematodes

Figure 3

Nile red staining of intestinal lipid droplets of C. elegans. Synchronized L1 were incubated in liquid S-medium with 200 µM SPT or DMSO (control) with and without nutrient source. After 48 h, the nematodes were fixed and stained with nile red and the number of lipid-depleted nematodes was counted. Only nematodes that were incubated with SPT-enol in the presence of a food source show intestinal lipid droplet depletion (92.3 ± 2.5% of total nematodes, average and SD, n = 3). Nematodes supplied with food and DMSO start to develop. The white bar represents 50 µm.

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