Figure 1
Epigenetic and transcriptomic changes of irradiated samples compared to non-irradiated controls: (a) Circos plot showing differential methylation (m, outer circle) and expression (e, inner circle) in response to irradiation to 0.9 MED in a genomic context (FDR < 0.05). Amplitude of points corresponds to log2 fold-change with the solid black line representing no change. Hypomethylated CpGs and downregulated genes are colored in blue, hypermethylated CpGs and upregulated genes in yellow. Colored bands in the karyogram mark centromeres (red) and heterochromatin status (grey to black). (b) Differential methylation of 49 genomic regions previously associated with chronic sun-exposure17 compared to differential methylation after acute repetitive irradiation. (c) Volcano plot of differential gene expression in response to irradiation. Differentially expressed genes with ≥ 3 differentially methylated CpGs are marked in red. (d) Genome-wide ratio of differentially up- and downregulated genes with concomitant change in methylation (≥ 3 CpGs). (e) Protein–protein-interaction network between the most interconnected differentially expressed and methylated genes. Points are scaled by the negative logarithmized FDR of differential expression and colored by log2 fold-changes. Edges are scaled by confidence of interaction. (f) Significantly correlated differential expression and enhancer methylation of CARD14, expression and TSS200 methylation of IRF8, expression and TSS200 methylation of CSNK2A2, and expression and TSS200 methylation of KRT17. Plots were generated using R v3.6.176 software.
