Figure 4
From: A FRET-based screening method to detect potential inhibitors of the binding of CNNM3 to PRL2
Inhibition of the binding of CNNM3-YPet and CyPet-PRL2 using non-CyPet-tagged PRL1 and PRL2. (A) Amino acid sequence alignment of human PRL1 (BAG70128.1) and human PRL2 (NP_001356788.1). Red boxes, identical residues; red letters, similar residues. (B) Structural comparison of PRL1 (blue, PDB: 1XM2) and PRL2 (orange, PDB: 5K22). (C) Non-CyPet PRL-based inhibition test of the binding of CNNM3-YPet (200 nM) and CyPet-PRL2 (200 nM) using FRET. All data are expressed as the mean ± SE. ***p < 0.001; ****p < 0.0001, by Tukey’s multiple comparison test, n = 6.
