Figure 6

Identification of multiple C16:1 FA isomers in human fibroblasts. (A) Mass chromatogram of C16:1 (m/z 253.22). The time points where each C16:1 isomer was identified are indicated by arrowheads. (B) Detected C16:1 isomers from the analysis. Three C16:1 isomers with double bonds at ω-7, ω-9, and ω-10 were discovered and their abundance ratio are shown. cis/trans isomerism is not considered. (C) Tandem mass spectra and diagnostic fragment structure of each C16:1 isomer at the indicated time points in (A) are shown and diagnostic fragment ions are indicated by asterisks.