Figure 7 | Scientific Reports

Figure 7

From: Positional determination of the carbon–carbon double bonds in unsaturated fatty acids mediated by solvent plasmatization using LC–MS

Figure 7

Identification and comparison of the C20:3 PUFA species between control fibroblasts and fibroblasts from a ZS patient. (A) Anti-catalase staining (green) of the fibroblasts. The nuclei were stained with DAPI in blue. Vesicular peroxisomes are visible only in the control fibroblast. (B) Mass chromatogram of C20:3 (m/z 305.25) where the peak tops are indicated by black (first peak) and white (second peak) arrowheads. (C) Tandem mass spectra at each peak where the diagnostic fragment spectra are indicated by asterisks. (D) Chemical structures and diagnostic fragmentation patterns of the detected C20:3 species are shown. Their relative abundances in PBD fibroblasts against control are shown in parentheses.

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