Table 2 Kidney and heart metabolites found to be most significantly correlated to anxiety-like behaviour.

Organ	Metabolite	r	p	Correlation
Kidney	Methionine.1	− 0.66	0.0007	Negative
	Malate.1	− 0.64	0.0011	Negative
	Malate.3	− 0.61	0.0019	Negative
	Glucose.1	0.60	0.0024	Positive
	2-Aminoadipate^‡	0.57	0.0042	Positive
	Cystine	0.56	0.0057	Positive
	Dimethylamine.1	0.54	0.0076	Positive
	Glutamate.1^‡, Methionine.2	− 0.53	0.0097	Negative
	3-Hydroxybutyrate.1	− 0.50	0.0150	Negative
	Glucose.3	0.49	0.0187	Positive
	Serine.1	0.45	0.0293	Positive
	Choline.5^‡	− 0.45	0.0307	Negative
	Choline.1^‡	− 0.44	0.0373	Negative
	Alanine^‡	0.43	0.0425	Positive
	Glucose.4	0.43	0.0397	Positive
Heart	2-Hydroxybutyrate.1	0.65	0.0009	Positive
	Alanine.3^‡, N-Phenylacetylglycine.2, O-Acetylcholine.1	− 0.63	0.0012	Negative
	3-Hydroxyisovalerate.2	0.63	0.0014	Positive
	Cholate.1	0.61	0.0019	Positive
	Formate	0.61	0.0018	Positive
	Galactitol.1	0.61	0.0020	Positive
	Methylmalonate.2	0.61	0.0022	Positive
	Thymol.7	0.61	0.0018	Positive
	4-Pyridoxate.1	0.60	0.0025	Positive
	Cholate.3	0.60	0.0025	Positive
	3-Methylglutarate.1	0.60	0.0022	Positive
	Methylmalonate.1	0.59	0.0033	Positive
	Alanine.4^‡, N-Phenylacetylglycine.4, O-Acetylcholine.2	− 0.59	0.0033	Negative
	Glutamate.8^‡	− 0.59	0.0033	Negative
	2-Aminoadipate.1^‡, Levulinate.1, Thymol.1, Valine.1	0.58	0.0038	Positive
	Malonate.1	0.57	0.0050	Positive
	Creatine	− 0.52	0.0114	Negative
	Lysine.1	0.49	0.0173	Positive

Pearson correlations were used to assess the relationship between behaviours indicative of heightened anxiety (i.e., more central squares entered in the open field) and relative concentrations of metabolites found to be significantly altered by stress in a Mann–Whitney U test (n = 9 control animals; n = 14 stressed animals). Top 15 r values, shown in descending order, correspond to Fig. 5. In hearts, the bottom three entries correspond to additional metabolites shown to have an association with adverse mental health outcomes. Positive correlations indicate that a higher anxiety-like state was linked to lower metabolite concentrations, while negative correlations indicate that a higher anxiety-like state was linked to higher metabolite concentrations. Metabolites for which more than one NMR resonance peak was identified are represented as metabolite.1, metabolite.2, … metabolite.n.
^‡Indicates metabolites that were significantly correlated to anxious behaviour in both kidney and heart tissues.

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