Figure 1

Oxygen consumption rate (OCR) measurements of purified mitochondria. (A) Simplified drawing of the electron transport chain located within the inner mitochondrial membrane. Electron transfer is coupled to the transfer of protons (H⁺) across the inner mitochondrial membrane into the inner membrane space, creating a proton gradient. This gradient is utilized by complex V for ATP synthesis. The protons react with oxygen to generate water. Thus, the OCR can be monitored by the Seahorse XF analyzers and used as a surrogate of mitochondrial respiration. The targets of the inhibitors (oligomycin, antimycin A, and rotenone) and uncoupler (FCCP) are indicated. (B) A representative OCR curve generated using isolated mitochondria showing the characteristic responses to mitochondrial inhibitors and the uncoupler FCCP.