Figure 2

Incubation of rat brain sections for various lengths of time prior to preparing tissue punches does not influence basal OCR. (A) Picture of the multi-segment storage chamber designed for maintaining tissues in oxygenated artificial cerebrospinal fluid. Each chamber contains brain sections from a separate animal. (B) Picture of a representative tissue section showing the location of the punches used to assess tissue viability. (C) Tissue punches need to be centered in the well prior to starting the assay to ensure accurate OCR measures. (D) Summary data showing basal respiration, monitored over time, in tissue sections rested for various lengths of time prior to the preparation of cortical punches. Data are presented as mean ± SEM.