Figure 6
ROCK inhibition selectively decreases the survival of ABC-DLBCL. (a) Viability analysis of BL, GCB-DLBCL, and ABC-DLBCL cell lines following 4 days treatment with 0 μM, 30 μM, 60 μM, or 90 μM Y-27 as determined by MTS proliferation assay (mean ± SEM; n =  > 2 per cell line; p value by 1-way ANOVA followed by Dunnett’s multiple comparisons test). (b) Representative histograms of propidium iodide (PI) incorporation showing the sub-G0 cell populations in DLBCL cells either left untreated or following 48 h treatment with 90 μM Y-27. Data representative of 3 independent experiments per cell line. (c) MTS proliferation assay of DLBCL cells following 4 days treatment with 90 μM Y-27 or 1 μM KD025 (mean ± SEM; n =  > 4 per cell line; p value by 1-way ANOVA followed by Dunnett’s multiple comparisons test). (d) Representative histograms of PI incorporation in DLBCL cells treated for 48 h with 90 μM Y-27 or 1 μM KD025. Data representative of 3 independent experiments per cell line. (e–f) U2932 (e) and HT (f) cells were established as a subcutaneous tumor in immunodeficient NSG mice and treated daily for 10–15 days with PBS (vehicle) or 40 mg/kg Y-27 by intraperitoneal injection. Tumor progression was monitored as a function of tumor volume. Data pooled from 8–10 mice per treatment condition per cell line. * p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.0001.
