Figure 1
From: Synthesis of glycoconjugates utilizing the regioselectivity of a lytic polysaccharide monooxygenase
A simple four step chemo-enzymatic procedure to generate a cello-oligosaccharide with FITC conjugated to C4 in the non-reducing end. The starting point is cellulose. NcLPMO9A cuts the cellulose chain and generates C4-oxidized cello-oligosaccharides. CDH converts the reducing end to its corresponding aldonic acid, which protects the reducing end from oxime formation. The oxime linker is coupled to the C-4 keto group prior to the last step in which FITC is cross-linked to the oxime linker.
