Figure 6
From: Synthesis of glycoconjugates utilizing the regioselectivity of a lytic polysaccharide monooxygenase
PGC-MS analysis of bi-functionalized cello-oligosaccharides. The oxime bi-functionalized oligosaccharides were separated by porous graphitized carbon (PGC) chromatography using an LTQ Velos Pro mass spectrometer (Thermo) for detection. (A) Overlay of three extracted ion chromatograms for m/z-ratios 619.25, 781.28 and 943.35, showing separation of the bi-functionalized products with DP 3, 4 and 5, respectively (note that these are proton adducts). The dashed line shows the time of recording of the MS/MS spectra. (B) MS/MS spectra of the three products show a dominant cleavage of the glycosidic bonds followed by multiple water losses (annotated as B-(H2O)2). A cleavage of the reducing-end amine linker bond can also be seen resulting in the following fragments; 543.17, 705.23 and 867.29 for the mother ions 619.25, 781.28 and 943.35, respectively. (C) Structures of the three bi-functionalized products, with theoretical masses and the observed fragmentation patterns (using the nomenclature of Domon and Costello)49.
