Figure 8

MALDI-ToF-MS analysis of a mixture of bi-functionalized aminooxy-cello-oligosaccharides and products formed after cellulase treatment of this mixture. The spectra shown are for an untreated mixture (magenta), a Cel5A treated mixture (red spectrum and red arrows; enzyme reaction 3) and a Cel6B treated mixture (green spectrum and green arrows; enzyme reaction 4). The insert shows the structure of the bi-functionalized pentamer, with solid and dotted arrows indicating the major and minor cleavage activity of Cel5A (red) and Cel6B (green), respectively; see text for details. Adducts of proton, sodium and potassium are labelled as [M+H]⁺, [M+Na]⁺ and [M+K]⁺, respectively. The spectra were generated with Flex analysis 3.4. https://www.bruker.com.