Figure 2 | Scientific Reports

Figure 2

From: Porcine reproductive and respiratory syndrome virus infection induces endoplasmic reticulum stress, facilitates virus replication, and contributes to autophagy and apoptosis

Figure 2

PRRSV infection activates the PERK signaling branch. Marc-145 cells were mock-infected or infected with PRRSV at an MOI of 0.5, and then harvested at the indicated time points. Cells treated with TG (5 μM) for 4 h, and collected at the indicated time points as the positive control. (A) Cell lysates were evaluated by western blot using antibodies specific for the phosphorylated forms of eIF2α, total eIF2α, Nsp2, and β-actin (loading control). The relative expression of p-eIF2α was analyzed with ImageJ based on the band intensities. Error bars indicate mean ± SD from three independent experiments. (B,C) RNA was extracted from cells at the indicated time points, and analyzed by quantitative RT-PCR using primers specific for ATF4 and GADD34. The fold changes of ATF4 and GADD34 were relative to mock-infected cells (set at 1). **p < 0.01.

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