Figure 5 | Scientific Reports

Figure 5

From: Porcine reproductive and respiratory syndrome virus infection induces endoplasmic reticulum stress, facilitates virus replication, and contributes to autophagy and apoptosis

Figure 5The alt text for this image may have been generated using AI.

UPR induction benefits PRRSV replication. (A) Marc-145 cells were treated with 4-PBA (5 mM), and infected with PRRSV for 36 h. GPR78 and GRP94 protein expression was evaluated by western blot. The relative expression was analyzed with ImageJ based on the band intensities. (B) Marc-145 cells were treated with 4-PBA, and infected with PRRSV. After that, cells were collected at the indicated time points, and the viral titers were determined by plaque assay. (C) Cells were pretreated with 4-PBA at different concentrations for 1 h, and then infected with PRRSV, 36 h later, virus proliferation was determined by plaque assay. (D,E) Marc-145 cells were transfected with the indicated siRNA. After 24 h, western blot was performed to analyze the expression of PERK and IRE-1. The relative expression of PERK and IRE1 was analyzed with ImageJ based on the band intensities. (F) Marc-145 cells were transfected with siRNAs targeting PERK or IRE-1 for 24 h, followed by PRRSV infection, Thirty-six hours post-infection, the cells were collected to determine viral replication by plaque assay. Results are expressed as mean ± SD for three independent experiments with three technical replicates per group. *p < 0.05 and **p < 0.01.

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