Figure 2
From: Immune outcomes of Zika virus infection in nonhuman primates
Monocyte transcriptional responses. (a) Heatmap showing patterns of antiviral gene expression in sorted CD14+ monocytes. Legend shows CD14+ (gray) and CD14− (white) fractions from the PBMCs of CD8-depleted and nondepleted animals, and fold change gene expression values were calculated using the NanoString platform for immune system related genes at 3 dpi relative to preinfection. The heatmap was generated using the pheatmap package (version 1.0.12, https://cran.r-project.org/web/packages/pheatmap/index.html) in R (version 3.6.1, https://cran.r-project.org/bin/windows/base/old/3.6.1/). (b,c) qPCR verification of antiviral gene expression in sorted CD14+ monocytes. Bar graphs shows the relative expression levels of an interferon stimulated gene (ISG15) and a pattern recognition related gene (DDX58) relative to b-actin in sorted CD14+ monocyte (b) and CD14− (c) fractions at the indicated timepoints (dpi, x-axis).
