Figure 6

BCG infection increased IL-10 production in IL-35-producing B cells. The production of IL-10 in B cells was determined by intracellular cytokine flow cytometry. PBMCs or LMC was extracted from BCG-infected mice (n = 10). Then, the frequencies of IL-10-producing in total B cells or in CD19⁺ p35⁺_B cells were analyzed by flow cytometry. The gating strategy is shown in Figure S3 (n = 10). (A, E) shows the representative histograms for flow cytometry analysis of the expression of IL-10 in B cells in PBMCs and B cells in LMCs (A), and the expression of IL-10 in p35 expressed B cells or in p35 nagative B cells in PBMCs (E) in BCG-infected mice and controls for 4 weeks. (B, C) Paired comparison of the frequency of IL-10-producing CD19⁺ B cells between blood and lung in controls (B) and BCG-infected mice (C). (D) Graph data showing the comparison of the frequency of IL-10-producing CD19⁺ B cells between BCG-infected mice and controls. (F, G) Paired comparison of the frequency of IL-10-positive cells between p35-expressing B cells and (F) p35 negative B cells (G) in PBMCs. (H) Graph data showing the comparison of the frequency of IL-10-producing cells in p35-expressing or in p35 negative B cells between BCG-infected mice and controls. The P value is shown in each column, *P < 0.05; **P < 0.01; and ***P < 0.001, or in each graph directly.